With entrance spirituality and God to management and Organization field, as 21st century challenge, organization’s managers and leaders especially educational institutions must confront with new phenomena. Spiritual leader’s role to incite and encourage human resource with use of spiritual vision and establishment of cultural context based on human values, to foster empowered, high productivity, committed, and motivated human resource. This paper explains spiritual leadership role on human resource empowerment. Population is included human resource that work in University of Tehran. The results of the research exhibit that there is a significant correlation between spiritual leadership in University of Tehran and human resource empowerment. Results indicate the significant and positive causal relationship between vision, altruistic love, faith, calling, membership, and negative causal relationship organizational commitment and significant and reverse causal relationship performance feedback with empowerment.